A Method for Measuring the Non-random Bias of a Codon Usage Table
A.D. McLachlan, R. Staden, D.R. Boswell
Nucleic Acids Research, 12(24):9567-9575 (Dec 21, 1984)
Abstract
We describe a new statistical method for measuring bias in the codon
usage table of a gene. The test is based on the multinomial and
Poisson distributions. The method is used to scan DNA sequences and
measure the strength of codon preference. For E. Coli we show that
the strength of codon preference is related to levels of gene
expression. The method can also be used to compare base triplet
frequencies with those expected from the base composition. This
second type of codon bias test is useful for distinguishing coding
from non-coding regions.
