An Integrated Transcript Map of Human Chromosome 1p35-p36
Sanford J. Jensen, Erik P. Sulman,
John M. Maris, Tara C. Matise,
Patrick J. Vojta, J. Carl Barrett, Garrett M.
Brodeur, Peter S. White
Genomics , Vol. 42, n.1, p126-136 (May 15, 1997)
Abstract
The distal short arm of human chromosome 1 (1p) is rearranged in a variety of
malignancies, and several genetic diseases also map to this region. We have
constructed an integrated transcript map to precisely define the positions of genes
and expressed sequence tags (ESTs) previously mapped to 1p35-p36, a region
spanning approximately 40 Mb. To anchor the integrated map, a framework
genetic map was constructed with 24 genetic markers and a marker order of
>1000:1 odds, yielding an average resolution of 2.8 cM. An additional 106 genetic
markers were localized relative to the framework genetic map. To place markers
more precisely within 1p35-p36, a chromosome 1-specific, radiation-reduced
hybrid (RH) panel was created. Individual DNA fragments of the RH panel were
identified and ordered by PCR with the framework genetic map. A total of 250
markers, including 142 genes and ESTs, were mapped by PCR against the RH
panel. The map has an observed resolution of 800 kb, and the results closely match
and more precisely define previous mapping information for most markers. This
map will help to identify candidate genes for genetic diseases mapping to distal 1p
and is fully integrated with existing genetic and RH maps of the human genome.
