A Radiation Hybrid Map of 506 STS Markers Spanning Human Chromosome 11
M.R. James, C.W. Richard 3rd, J.J. Schott, C. Yousry K. Clark,
J. Bell, J.D. Terwilliger, J. Hazan, C. Dubay, A. Vignal,
M. Agrapart, T. Imai, Y. Nakamura, M. Polymeropoulos,
J. Weissenbach, D.R. Cox, G.M. Lathrop
Nature Genetics , 8(1), 70--76 (1994 Sep)
Abstract
We present a high resolution radiation hybrid map of human
chromosome 11 using 506 sequence tagged sites (STSs) scored on a
panel of 86 radiation hybrids. The 506 STSs fall into 299 unique
positions (average resolution of about 480 kilobases (kb)) that span
the whole chromosome. A subset of 260 STSs (143 positions) form a
framework map that has a resolution of approximately 1 megabase
between adjacent positions and is ordered with odds of at least
1,000:1. The centromere was clearly defined with pericentric markers
unambiguously assigned to the short or long arm. The map contains
most genes (125) and expressed sequence tags (26) currently assigned
to chromosome 11 and more than half of the STSs are polymorphic
microsatellite loci. These markers and the map can be used for high
resolution physical and genetic mapping.
