A Microsatellite Genetic Linkage Map of Human Chromosome 13
K.E. Petrukhin, M.C. Speer, E. Cayanis,
M. de Fatima Bonaldo, U. Tantravahi, M.B. Soares,
S.G. Fischer, D. Warburton, T.C. Gilliam, J. Ott
Genomics , 15(1),76--85 (1993 Jan)
Abstract
We have characterized 21 polymorphic (CA)n microsatellites for the
development of a genetic map of chromosome 13. Fifteen markers were
isolated from a flow-sorted chromosome 13 library, four CA repeats
were derived from NotI-containing cosmid clones, and two polymorphic
markers were described previously (J. L. Weber, A. E. Kwitek, and P.
E. May, 1990, Nucleic Acids Res. 18: 4638; L. Warnich, I. Groenwald,
L. Laubscher, and A. E. Retief, 1991, Am. J. Hum. Genet. 49(Suppl.):
372 (Abstract)). Regional localization for all of the markers was
performed by amplification of DNA from five somatic cell hybrids
containing different deletions of chromosome 13. Genetic markers
were shown to be distributed throughout 6 of the 11 resolvable
chromosomal subregions. Using data from nine families provided by
the Centre d'Etude du Polymorphisme Humain (CEPH), a framework map
of 12 of these 21 markers was developed. Six of the 12 markers form
three pairs, with each two members of a pair being tightly linked,
such that nine systems of markers can be distinguished. The average
heterozygosity of these 12 markers is 0.75. The total length of the
sex-averaged map is 65.4 cM (Kosambi), with an average distance of
8.2 cM between systems of markers (eight intervals). Seven remaining
markers were placed provisionally into the framework map.
