Localization of the Gene for the Wiskott-Aldrich Syndrome Between
Two Flanking Markers, TIMP and DXS255, on Xp11.22-Xp11.3
S-P. Kwan, T. Lehner , T. Hagemann, B. Lu, M. Blaese,
H. Ochs, R. Wedgwood, J. Ott, I.W. Craig, F.S. Rosen
Genomics , 10(1), 29--33 (1991 May)
Abstract
The Wiskott-Aldrich syndrome (WAS) is an X-linked recessive genetic
disease in which the basic molecular defect is unknown. We
previously located the WAS gene between two DNA markers, DXS7
(Xp11.3) and DXS14 (Xp11), and mapped it to the proximal short arm
of the human X chromosome (Kwan et al., 1988, Genomics 3:39-43). In
this study, further mapping was performed on 17 WAS families with
two additional RFLP markers, TIMP and DXS255. Our data suggest that
DXS255 is closer to the WAS locus than any other markers that have
been previously described, with a multipoint maximum lod score of Z
= 8.59 at 1.2 cM distal to DXS255 and thus further refine the
position of the WAS gene on the short arm of the X chromosome.
Possible locations for the WAS gene are entirely confined between
TIMP (Xp11.3) and DXS255 (Xp11.22). Use of these markers thus
represents a major improvement in genetic prediction in WAS
families.
