Extreme discordant sib pairs for mapping quantitative trait loci in humans
N. Risch and H. Zhang
Science, 268(5217),1584-1589 (Jun 16, 1995)
Abstract
Analysis of differences between siblings (sib pair analysis) is a
standard method of genetic linkage analysis for mapping quantitative
trait loci, such as those contributing to hypertension and obesity,
in humans. In traditional designs, pairs are selected at random or
with one sib having an extreme trait value. The majority of such
pairs provide little power to detect linkage; only pairs that are
concordant for high values, low values, or extremely discordant
pairs (for example, one in the top 10 percent and the other in the
bottom 10 percent of the distribution) provide substantial power.
Focus on discordant pairs can reduce the amount of genotyping
necessary over conventional designs by 10- to 40-fold.
Allele-sharing linkage analysis